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Following the emergence of the SARS-CoV-2 (Covid-19) pandemic, interest in understanding antibody diagnostic testing has increased. We describe a quick and inexpensive technique that enabled students to print their own microfluidic devices that can be used to house an immunoassay for detecting a Human Immunodeficiency Virus (HIV) antibody. Both qualitative diagnostic assays and quantitative binding assays were carried out to characterize the HIV interaction with a target antibody. By performing these hands-on low-cost experiments in the analytical chemistry lab course, students were exposed to 3D fabrication, microfluidic technology, surface chemistry, protein-ligand binding affinity studies, and immunoassays within the time frame of two four–hour laboratory periods.more » « less
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